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Integrated thin-film silicon chemiluminescence sensors for protein detection
A hydrogenated amorphous silicon photodiode is used for the integrated, real-time detection of chemiluminescent reactions in solution. The light emission at 425 nm resulting from the horseradish peroxidase-catalyzed oxidation of luminol is detected by a p-i-n amorphous silicon photodiode operated at zero bias. The sensitivity achieved is in the range of nanomole of horseradish peroxidase per liter.
Integrated chemiluminescence sensor using a p-i-n a-Si:H photodiode. So far, the minimum detected concentration of HRP was 2.4 nmol/L.